Part:BBa_M36855
Recombinase-based heat-inducible sensor
This part is a recombinase-based heat-inducible sensor. Under high-temperature conditions, RNA polymerase binds to the heat shock promoter (HSP) and begins transcription. The translated Bxb1 integrase protein then flips the DNA region flanked by recombinase sites attB and attP, irreversibly flipping a constitutive promoter to begin transcription of whatever downstream actuator is placed after the part.
Usage and Biology
The part serves as a binary memory device to record whether or not the organism has ever been exposed to temperatures above a certain threshold and can be coupled to the output of the user's choice. A potential application of the technology would be in a temperature sensor for food items that may be sensitive to exposure to high temperatures. An easy detected readout (GFP, RFP) could signal to the user or purveyor when a good has been exposed to high temperatures (perhaps sitting in a warm truck if refrigeration failed) and alert them to potential food safety concerns. A well-characterized heat-inducible promoter was used so that there should be a clear difference in expression from 4 C (refrigeration temperatures) to 37 C and above.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1545
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1765
Illegal NheI site found at 1788
Illegal PstI site found at 1545 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1545
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1545
Illegal NgoMIV site found at 1712
Illegal AgeI site found at 1599 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1732
Illegal BsaI.rc site found at 1815
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